Molecular characterization of enterotoxin genes in methicillin-resistant S. aureus isolated from food poisoning outbreaks in Egypt

Table 1 Primers used in this work to detect mecA and Staphylococcal enterotoxin genes

Primer name	Primer sequence	Annealing temp. (°C)	Size (bp)	Citations
Mec-A
F	GTAGAAATGACTGAACGTCCGATAA	52	310	[29]
R	CCAATTCCACATTGTTCGGTCTAA
Set-A
F	GGATATTGTTGATAAATATAAAGGGAAAAAAG	53	439	[30]
R	GTTAATCGTTTTATTATCTCTATATATTCTTAATAGT
Oligo	GTTTCATACTTCTACAGAACCTTC
Set-B
F	AGATTTAGCTGATAAATACAAAGATAAATACG	54	494	[30]
R	TCGTAAGATAAACTTCAATCTTCACATCT
Oligo	AAACTCTATGAATTTAACAACTCG
Set-C
F	AGATTTAGCAAAGAAGTACAAAGATG	53	490	[30]
R	AAGGTGGACTTCTATCTTCACACTT
Oligo	AACCACTTTGATAATGGGAACTTA
Set-D
F	AGATTTAGCAAAGAAGTACAAAGATG	53	481	[30]
R	CTACTTTTCATATAAATAGATGTCAATATG
Oligo	TCAATTTGTGGATAAATGGTGTAC
Set-E
F	AGATTTAGCAAAGAAGTACAAAGATG	54	473	[30]
R	TGTATAAATACAAATCAATATGGAGGTTCTCT
Oligo	TGGTTTATATAACTCAGACAGCTTT
Set-G
F	AGAATTAGCTAACAATTATAAAGATAAAAAAG	52	496	[30]
R	TCAGTGAGTATTAAGAAATACTTCCAT
Oligo	AACAATCGACAATAGACAATCACT
Set-I
F	TGATTTAGCTCAGAAGTTTAAAAATAAAAATG	52	505	[30]
R	TTAGTTACTATCTACATATGATATTTCGA
Oligo	AGGCAAAGAATATGGATATAAATCT
Set-M
F	ATGAAAAGAATACTTATCATTGTTGTTTTATTG	53	720	[30]
R	CTTCAACTTTCGTCCTTATAAGATATTTC
Oligo	AACAGGACAAGCTGAAAGTTTC
Set-N
F	ATAAAAAATATTAAAAAGCTTATGAGATTGTTC	51	777	[30]
R	ACTTAATCTTTATATAAAAATACATCAATATG
Oligo	ATACAATAAAGATACCGGTAACAT
Set-O
F	TATGTAGTGTAAACAATGCATATGCA	53	685	[30]
R	TCTATTGTTTTATTATCATTATAAATTTGCAAAT
Oligo	ATGACAGAATGACTAGTGATGTA

ISSN: 2072-1315